Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells
Joint Authors
Nielsen, Ditte Søvsø Gundelund
Fredborg, Marlene
Andersen, Vibeke
Purup, Stig
Source
International Journal of Inflammation
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Inflammatory bowel diseases (IBD) are chronic inflammatory diseases involving all or part of the gastrointestinal tract.
The stress-activated serine-threonine protein kinase D1 (PKD1) protein has previously been implicated in intestinal immune regulation.
The objective of this study was to evaluate the effects of human PKD1 in relation to intestinal inflammation, using a co-culture model of intestinal epithelial Caco-2 cells and RAW264.7 macrophages.
An inflammatory response was induced in the macrophages by lipopolysaccharide (LPS), upregulating the expression of tumour necrosis factor alpha (TNF-α), interleukin- (IL-) 1β, and IL-6 besides increasing the secretion of TNF-α protein.
The effect of administering PKD1 to Caco-2 was evaluated in relation to both amelioration of inflammation and the ability to suppress inflammation initiation.
Administration of PKD1 (10–100 ng/ml) following induction of inflammation induced downregulation of TNF-α expression in RAW264.7 cells.
In addition, PKD1 administered for 3 h prior to LPS stimulation reduced the subsequent inflammatory response through downregulation of TNF-α, IL-1β, and IL-6 in RAW264.7 cells.
These results demonstrate a potential role of PKD1 in the intercellular communication between intestinal epithelial and immune cells, proposing a protective effect of PKD1 on the induction of an inflammatory response in macrophages, an important aspect during the pathogenesis of IBD.
American Psychological Association (APA)
Nielsen, Ditte Søvsø Gundelund& Fredborg, Marlene& Andersen, Vibeke& Purup, Stig. 2017. Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells. International Journal of Inflammation،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1167507
Modern Language Association (MLA)
Nielsen, Ditte Søvsø Gundelund…[et al.]. Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells. International Journal of Inflammation No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1167507
American Medical Association (AMA)
Nielsen, Ditte Søvsø Gundelund& Fredborg, Marlene& Andersen, Vibeke& Purup, Stig. Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells. International Journal of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1167507
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167507