An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib
المؤلفون المشاركون
Zhao, Fang
Ying, Zhenzhen
Wei, Jingyao
Liu, Ruijuan
Yu, Yifang
Tian, Xin
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Osimertinib is a novel oral, potent, and irreversible epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) for treatment of advanced T790M mutation-positive advanced non-small cell lung cancer, which is commonly combined with ginsenoside Rg3 in clinic to enhance the efficacy and minimize adverse reactions.
In the present study, a highly sensitive UPLC-MS/MS method was established and validated for analysis of osimertinib in rat plasma according to US FDA guideline.
Separation was performed on a C18 (2.1 × 50 mm, 2.6 μm) column using a gradient elution of ammonium formate (10 mM) with 0.1% formic acid buffer (A) and ACN (B) at a flow rate of 0.2 mL/min.
Detection was carried out on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization in the MRM mode.
The method was validated over a concentration range of 1–400 ng/mL for osimertinib.
The intra- and interday accuracy and precision values were within ±15%.
No significant degradation occurred under the experimental conditions in stability assays.
There was a further investigation on the effects of multiple doses of ginsenoside Rg3 on the pharmacokinetics of osimertinib in rats for the first time.
The results implied that osimertinib exhibited a slow absorption and moderate-rate elimination in rats following oral administration.
Coadministeration with ginsenoside Rg3 (5 mg/kg, 7 days, i.g.) may have no effect on the pharmacokinetics of osimertinib in rats.
The results provide a reference for the clinical concomitant medications of Rg3 and osimertinib.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ying, Zhenzhen& Wei, Jingyao& Liu, Ruijuan& Zhao, Fang& Yu, Yifang& Tian, Xin. 2020. An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167732
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ying, Zhenzhen…[et al.]. An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167732
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ying, Zhenzhen& Wei, Jingyao& Liu, Ruijuan& Zhao, Fang& Yu, Yifang& Tian, Xin. An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167732
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167732
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر