An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib
Joint Authors
Zhao, Fang
Ying, Zhenzhen
Wei, Jingyao
Liu, Ruijuan
Yu, Yifang
Tian, Xin
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Osimertinib is a novel oral, potent, and irreversible epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) for treatment of advanced T790M mutation-positive advanced non-small cell lung cancer, which is commonly combined with ginsenoside Rg3 in clinic to enhance the efficacy and minimize adverse reactions.
In the present study, a highly sensitive UPLC-MS/MS method was established and validated for analysis of osimertinib in rat plasma according to US FDA guideline.
Separation was performed on a C18 (2.1 × 50 mm, 2.6 μm) column using a gradient elution of ammonium formate (10 mM) with 0.1% formic acid buffer (A) and ACN (B) at a flow rate of 0.2 mL/min.
Detection was carried out on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization in the MRM mode.
The method was validated over a concentration range of 1–400 ng/mL for osimertinib.
The intra- and interday accuracy and precision values were within ±15%.
No significant degradation occurred under the experimental conditions in stability assays.
There was a further investigation on the effects of multiple doses of ginsenoside Rg3 on the pharmacokinetics of osimertinib in rats for the first time.
The results implied that osimertinib exhibited a slow absorption and moderate-rate elimination in rats following oral administration.
Coadministeration with ginsenoside Rg3 (5 mg/kg, 7 days, i.g.) may have no effect on the pharmacokinetics of osimertinib in rats.
The results provide a reference for the clinical concomitant medications of Rg3 and osimertinib.
American Psychological Association (APA)
Ying, Zhenzhen& Wei, Jingyao& Liu, Ruijuan& Zhao, Fang& Yu, Yifang& Tian, Xin. 2020. An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167732
Modern Language Association (MLA)
Ying, Zhenzhen…[et al.]. An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167732
American Medical Association (AMA)
Ying, Zhenzhen& Wei, Jingyao& Liu, Ruijuan& Zhao, Fang& Yu, Yifang& Tian, Xin. An UPLC-MSMS Method for Determination of Osimertinib in Rat Plasma: Application to Investigating the Effect of Ginsenoside Rg3 on the Pharmacokinetics of Osimertinib. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167732
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167732