Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis
المؤلفون المشاركون
Ouyang, Lizhi
Li, Hui
Zhou, Suping
Johnson, Terrance
Vercruysse, Koen
Ranganathan, Parthasarathy
Phambu, Nsoki
Ropelewski, Alexander J.
Thannhauser, Theodore W.
المصدر
International Journal of Polymer Science
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-10
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In previous work, we reported on the isolation and genome sequence analysis of Bacillus cereus strain tsu1 NCBI accession number JPYN00000000.
The 36 scaffolds in the assembled tsu1 genome were all aligned with B.
cereus B4264 genome with variations.
Genes encoding for xylanase and cellulase and the cluster of genes in the poly-3-hydroxybutyrate (PHB) biosynthesis pathway were identified in tsu1 genome.
The PHB accumulation in B.
cereus tsu1 was initially identified using Sudan Black staining and then confirmed using high-performance liquid chromatography.
Physical properties of these PHB extracts, when analyzed with Raman spectra and Fourier transform infrared spectroscopy, were found to be comparable to the standard compound.
The five PHB genes in tsu1 (phaA, phaB, phaR, phaC, and phaP) were cloned and expressed with TOPO cloning, and the recombinant proteins were validated using peptide mapping of in-gel trypsin digestion followed by mass spectrometry analysis.
The recombinant E.
coli BL21 (DE3) (over)expressing phaC was found to accumulate PHB particles.
The cellulolytic activity of tsu1 was detected using carboxymethylcellulose (CMC) plate Congo red assay and the shift towards low-molecular size forms of CMC revealed by gel permeation chromatography in CMC liquid culture and the identification of a cellulase in the secreted proteome.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Hui& Zhou, Suping& Johnson, Terrance& Vercruysse, Koen& Ouyang, Lizhi& Ranganathan, Parthasarathy…[et al.]. 2017. Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis. International Journal of Polymer Science،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1169086
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Hui…[et al.]. Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis. International Journal of Polymer Science No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1169086
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Hui& Zhou, Suping& Johnson, Terrance& Vercruysse, Koen& Ouyang, Lizhi& Ranganathan, Parthasarathy…[et al.]. Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis. International Journal of Polymer Science. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1169086
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169086
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر