Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis
Joint Authors
Ouyang, Lizhi
Li, Hui
Zhou, Suping
Johnson, Terrance
Vercruysse, Koen
Ranganathan, Parthasarathy
Phambu, Nsoki
Ropelewski, Alexander J.
Thannhauser, Theodore W.
Source
International Journal of Polymer Science
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In previous work, we reported on the isolation and genome sequence analysis of Bacillus cereus strain tsu1 NCBI accession number JPYN00000000.
The 36 scaffolds in the assembled tsu1 genome were all aligned with B.
cereus B4264 genome with variations.
Genes encoding for xylanase and cellulase and the cluster of genes in the poly-3-hydroxybutyrate (PHB) biosynthesis pathway were identified in tsu1 genome.
The PHB accumulation in B.
cereus tsu1 was initially identified using Sudan Black staining and then confirmed using high-performance liquid chromatography.
Physical properties of these PHB extracts, when analyzed with Raman spectra and Fourier transform infrared spectroscopy, were found to be comparable to the standard compound.
The five PHB genes in tsu1 (phaA, phaB, phaR, phaC, and phaP) were cloned and expressed with TOPO cloning, and the recombinant proteins were validated using peptide mapping of in-gel trypsin digestion followed by mass spectrometry analysis.
The recombinant E.
coli BL21 (DE3) (over)expressing phaC was found to accumulate PHB particles.
The cellulolytic activity of tsu1 was detected using carboxymethylcellulose (CMC) plate Congo red assay and the shift towards low-molecular size forms of CMC revealed by gel permeation chromatography in CMC liquid culture and the identification of a cellulase in the secreted proteome.
American Psychological Association (APA)
Li, Hui& Zhou, Suping& Johnson, Terrance& Vercruysse, Koen& Ouyang, Lizhi& Ranganathan, Parthasarathy…[et al.]. 2017. Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis. International Journal of Polymer Science،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1169086
Modern Language Association (MLA)
Li, Hui…[et al.]. Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis. International Journal of Polymer Science No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1169086
American Medical Association (AMA)
Li, Hui& Zhou, Suping& Johnson, Terrance& Vercruysse, Koen& Ouyang, Lizhi& Ranganathan, Parthasarathy…[et al.]. Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis. International Journal of Polymer Science. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1169086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169086