Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature
المؤلفون المشاركون
Tai, Yanni
Zou, Fuxian
Zhang, Qiurong
Wang, Jia
Rao, Ronghui
Xie, Ruihua
Wu, Shuisheng
Chu, Kedan
Xu, Wen
Li, Xiaoyan
Huang, Mingqing
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-14
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Rhizoma Alismatis (RA), widely known as “Ze-Xie” in China, is the tuber of Alisma orientale (Sam.) Juzep (Alismaceae), a Chinese herbal medicine that has been used to treat hyperlipidemia, diabetes, hypertension, dysuria, and inflammation.
In this study, a sensitive and reliable method based on an ultra-performance liquid chromatography (UPLC) couple with two ionisation modes, including electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) tandem mass spectrometry (MS), namely, UPLC-ESI/APCI-MS/MS was developed and validated to simultaneously determine 8 triterpenoids (ESI mode) and 2 sesquiterpenoids (APCI mode) in RA.
Ten marker compounds were analysed with a Waters’ CORTECS UPLC C18 column (200 mm × 2.1 m, 1.6 μm) and gradient elution with water (contained 0.1% formic) and acetonitrile within 7 min.
The established method was validated for linearity, intra- and interday precisions, accuracy, recovery, and stability.
The calibration curve for 10 marker compounds showed good linear regression (r > 0.9971).
The limits of detection and quantification for analytes were 0.14–1.67 ng/mL and 0.44–5.65 ng/mL, respectively.
The relative standard deviations (RSD, %) and accuracy (RE, %) of intra- and interday precisions were less than 3.83% and 1.21% and 3.22% and 1.46%, repeatability and stability for real samples were less than 2.78% and 3.19%, respectively.
All recoveries of the 10 marker compounds ranged from 97.24% to 102.49% with RSDs less than 4.05%.
The developed method efficiently determined the 10 marker compounds in RA and was subsequently applied to optimise harvest time and crude processing temperature.
The result indicated the 90% wilted phase and 70°C (or lower) may be the best harvest time and the processing temperature of RA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tai, Yanni& Zou, Fuxian& Zhang, Qiurong& Wang, Jia& Rao, Ronghui& Xie, Ruihua…[et al.]. 2019. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1169327
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tai, Yanni…[et al.]. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1169327
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tai, Yanni& Zou, Fuxian& Zhang, Qiurong& Wang, Jia& Rao, Ronghui& Xie, Ruihua…[et al.]. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1169327
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169327
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر