Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature
Joint Authors
Tai, Yanni
Zou, Fuxian
Zhang, Qiurong
Wang, Jia
Rao, Ronghui
Xie, Ruihua
Wu, Shuisheng
Chu, Kedan
Xu, Wen
Li, Xiaoyan
Huang, Mingqing
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-14
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Rhizoma Alismatis (RA), widely known as “Ze-Xie” in China, is the tuber of Alisma orientale (Sam.) Juzep (Alismaceae), a Chinese herbal medicine that has been used to treat hyperlipidemia, diabetes, hypertension, dysuria, and inflammation.
In this study, a sensitive and reliable method based on an ultra-performance liquid chromatography (UPLC) couple with two ionisation modes, including electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) tandem mass spectrometry (MS), namely, UPLC-ESI/APCI-MS/MS was developed and validated to simultaneously determine 8 triterpenoids (ESI mode) and 2 sesquiterpenoids (APCI mode) in RA.
Ten marker compounds were analysed with a Waters’ CORTECS UPLC C18 column (200 mm × 2.1 m, 1.6 μm) and gradient elution with water (contained 0.1% formic) and acetonitrile within 7 min.
The established method was validated for linearity, intra- and interday precisions, accuracy, recovery, and stability.
The calibration curve for 10 marker compounds showed good linear regression (r > 0.9971).
The limits of detection and quantification for analytes were 0.14–1.67 ng/mL and 0.44–5.65 ng/mL, respectively.
The relative standard deviations (RSD, %) and accuracy (RE, %) of intra- and interday precisions were less than 3.83% and 1.21% and 3.22% and 1.46%, repeatability and stability for real samples were less than 2.78% and 3.19%, respectively.
All recoveries of the 10 marker compounds ranged from 97.24% to 102.49% with RSDs less than 4.05%.
The developed method efficiently determined the 10 marker compounds in RA and was subsequently applied to optimise harvest time and crude processing temperature.
The result indicated the 90% wilted phase and 70°C (or lower) may be the best harvest time and the processing temperature of RA.
American Psychological Association (APA)
Tai, Yanni& Zou, Fuxian& Zhang, Qiurong& Wang, Jia& Rao, Ronghui& Xie, Ruihua…[et al.]. 2019. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1169327
Modern Language Association (MLA)
Tai, Yanni…[et al.]. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1169327
American Medical Association (AMA)
Tai, Yanni& Zou, Fuxian& Zhang, Qiurong& Wang, Jia& Rao, Ronghui& Xie, Ruihua…[et al.]. Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESIAPCI-MSMS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1169327
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169327