Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials
المؤلفون المشاركون
Seifalian, Alexander Marcus
Naderi, Naghmeh
Kalaskar, Deepak M.
Malins, Edward
Becer, Remzi
Thornton, Catherine A.
Whitaker, Iain S.
Mosahebi, Ash
Butler, Peter E. M.
Griffin, Michelle
المصدر
International Journal of Biomaterials
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-10-03
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
An effective sterilisation technique that maintains structure integrity, mechanical properties, and biocompatibility is essential for the translation of new biomaterials to the clinical setting.
We aimed to establish an effective sterilisation technique for a biodegradable (POSS-PCL) and nonbiodegradable (POSS-PCU) nanocomposite scaffold that maintains stem cell biocompatibility.
Scaffolds were sterilised using 70% ethanol, ultraviolet radiation, bleach, antibiotic/antimycotic, ethylene oxide, gamma irradiation, argon plasma, or autoclaving.
Samples were immersed in tryptone soya broth and thioglycollate medium and inspected for signs of microbial growth.
Scaffold surface and mechanical and molecular weight properties were investigated.
AlamarBlue viability assay of adipose derived stem cells (ADSC) seeded on scaffolds was performed to investigate metabolic activity.
Confocal imaging of rhodamine phalloidin and DAPI stained ADSCs was performed to evaluate morphology.
Ethylene oxide, gamma irradiation, argon plasma, autoclaving, 70% ethanol, and bleach were effective in sterilising the scaffolds.
Autoclaving, gamma irradiation, and ethylene oxide led to a significant change in the molecular weight distribution of POSS-PCL and gamma irradiation and ethylene oxide to that of POSS-PCU (p<0.05).
UV, ethanol, gamma irradiation, and ethylene oxide caused significant changes in the mechanical properties of POSS-PCL (p<0.05).
Argon was associated with significantly higher surface wettability and ADSC metabolic activity (p<0.05).
In this study, argon plasma was an effective sterilisation technique for both nonbiodegradable and biodegradable nanocomposite scaffolds.
Argon plasma should be further investigated as a potential sterilisation technique for medical devices.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Griffin, Michelle& Naderi, Naghmeh& Kalaskar, Deepak M.& Malins, Edward& Becer, Remzi& Thornton, Catherine A.…[et al.]. 2018. Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials. International Journal of Biomaterials،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1169589
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Griffin, Michelle…[et al.]. Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials. International Journal of Biomaterials No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1169589
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Griffin, Michelle& Naderi, Naghmeh& Kalaskar, Deepak M.& Malins, Edward& Becer, Remzi& Thornton, Catherine A.…[et al.]. Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials. International Journal of Biomaterials. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1169589
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169589
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر