Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials
Joint Authors
Seifalian, Alexander Marcus
Naderi, Naghmeh
Kalaskar, Deepak M.
Malins, Edward
Becer, Remzi
Thornton, Catherine A.
Whitaker, Iain S.
Mosahebi, Ash
Butler, Peter E. M.
Griffin, Michelle
Source
International Journal of Biomaterials
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-03
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
An effective sterilisation technique that maintains structure integrity, mechanical properties, and biocompatibility is essential for the translation of new biomaterials to the clinical setting.
We aimed to establish an effective sterilisation technique for a biodegradable (POSS-PCL) and nonbiodegradable (POSS-PCU) nanocomposite scaffold that maintains stem cell biocompatibility.
Scaffolds were sterilised using 70% ethanol, ultraviolet radiation, bleach, antibiotic/antimycotic, ethylene oxide, gamma irradiation, argon plasma, or autoclaving.
Samples were immersed in tryptone soya broth and thioglycollate medium and inspected for signs of microbial growth.
Scaffold surface and mechanical and molecular weight properties were investigated.
AlamarBlue viability assay of adipose derived stem cells (ADSC) seeded on scaffolds was performed to investigate metabolic activity.
Confocal imaging of rhodamine phalloidin and DAPI stained ADSCs was performed to evaluate morphology.
Ethylene oxide, gamma irradiation, argon plasma, autoclaving, 70% ethanol, and bleach were effective in sterilising the scaffolds.
Autoclaving, gamma irradiation, and ethylene oxide led to a significant change in the molecular weight distribution of POSS-PCL and gamma irradiation and ethylene oxide to that of POSS-PCU (p<0.05).
UV, ethanol, gamma irradiation, and ethylene oxide caused significant changes in the mechanical properties of POSS-PCL (p<0.05).
Argon was associated with significantly higher surface wettability and ADSC metabolic activity (p<0.05).
In this study, argon plasma was an effective sterilisation technique for both nonbiodegradable and biodegradable nanocomposite scaffolds.
Argon plasma should be further investigated as a potential sterilisation technique for medical devices.
American Psychological Association (APA)
Griffin, Michelle& Naderi, Naghmeh& Kalaskar, Deepak M.& Malins, Edward& Becer, Remzi& Thornton, Catherine A.…[et al.]. 2018. Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials. International Journal of Biomaterials،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1169589
Modern Language Association (MLA)
Griffin, Michelle…[et al.]. Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials. International Journal of Biomaterials No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1169589
American Medical Association (AMA)
Griffin, Michelle& Naderi, Naghmeh& Kalaskar, Deepak M.& Malins, Edward& Becer, Remzi& Thornton, Catherine A.…[et al.]. Evaluation of Sterilisation Techniques for Regenerative Medicine Scaffolds Fabricated with Polyurethane Nonbiodegradable and Bioabsorbable Nanocomposite Materials. International Journal of Biomaterials. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1169589
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169589