Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells
المؤلفون المشاركون
Zhang, Hao
Dong, Qi
Zhang, Ping
Wang, Zhihong
Qin, Yuan
Wu, Changhao
Dong, Wenwu
المصدر
International Journal of Endocrinology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-12
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy.
Adipose-derived stem cells can undergo adipogenic and osteogenic differentiation in vitro and are considered a novel source of parathyroid-like cells, but the idea lacks theoretical basis and feasibility.
We aimed at establishing a protocol for differentiating adipose-derived stem cells into parathyroid-like cells for treating hypoparathyroidism.
Materials/Methods.
Adipose-derived stem cells were isolated and purified from the inguinal adipose tissue of Sprague Dawley rats.
Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively.
The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A.
The differentiation of the adipose-derived stem cells to parathyroid-like cells was confirmed by the detection of parathyroid hormone and the related parathyroid markers.
Results.
Adipose-derived stem cells were successfully isolated and purified from the rat adipocytes.
The adipogenic and osteogenic differentiation capabilities of the adipose-derived stem cells were determined.
SHH and activin A stimulated parathyroid hormone secretion by the adipose-derived stem cells and significantly increased the expression of calcium-sensing receptor (CaSR), parathyroid hormone, and glial cells missing homolog 2 (GCM2) in the cells in a time- and concentration-dependent manner.
Conclusion.
We successfully differentiated rat adipose-derived stem cells into parathyroid-like cells, which will pave a new route to curing hypoparathyroidism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ping& Zhang, Hao& Dong, Wenwu& Wang, Zhihong& Qin, Yuan& Wu, Changhao…[et al.]. 2020. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ping…[et al.]. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ping& Zhang, Hao& Dong, Wenwu& Wang, Zhihong& Qin, Yuan& Wu, Changhao…[et al.]. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1170124
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر