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Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells
المؤلفون المشاركون
Zhang, Hao
Dong, Qi
Zhang, Ping
Wang, Zhihong
Qin, Yuan
Wu, Changhao
Dong, Wenwu
المصدر
International Journal of Endocrinology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-12
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Background.
The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy.
Adipose-derived stem cells can undergo adipogenic and osteogenic differentiation in vitro and are considered a novel source of parathyroid-like cells, but the idea lacks theoretical basis and feasibility.
We aimed at establishing a protocol for differentiating adipose-derived stem cells into parathyroid-like cells for treating hypoparathyroidism.
Materials/Methods.
Adipose-derived stem cells were isolated and purified from the inguinal adipose tissue of Sprague Dawley rats.
Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively.
The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A.
The differentiation of the adipose-derived stem cells to parathyroid-like cells was confirmed by the detection of parathyroid hormone and the related parathyroid markers.
Results.
Adipose-derived stem cells were successfully isolated and purified from the rat adipocytes.
The adipogenic and osteogenic differentiation capabilities of the adipose-derived stem cells were determined.
SHH and activin A stimulated parathyroid hormone secretion by the adipose-derived stem cells and significantly increased the expression of calcium-sensing receptor (CaSR), parathyroid hormone, and glial cells missing homolog 2 (GCM2) in the cells in a time- and concentration-dependent manner.
Conclusion.
We successfully differentiated rat adipose-derived stem cells into parathyroid-like cells, which will pave a new route to curing hypoparathyroidism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ping& Zhang, Hao& Dong, Wenwu& Wang, Zhihong& Qin, Yuan& Wu, Changhao…[et al.]. 2020. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ping…[et al.]. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ping& Zhang, Hao& Dong, Wenwu& Wang, Zhihong& Qin, Yuan& Wu, Changhao…[et al.]. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1170124
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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