Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells
Joint Authors
Zhang, Hao
Dong, Qi
Zhang, Ping
Wang, Zhihong
Qin, Yuan
Wu, Changhao
Dong, Wenwu
Source
International Journal of Endocrinology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy.
Adipose-derived stem cells can undergo adipogenic and osteogenic differentiation in vitro and are considered a novel source of parathyroid-like cells, but the idea lacks theoretical basis and feasibility.
We aimed at establishing a protocol for differentiating adipose-derived stem cells into parathyroid-like cells for treating hypoparathyroidism.
Materials/Methods.
Adipose-derived stem cells were isolated and purified from the inguinal adipose tissue of Sprague Dawley rats.
Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively.
The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A.
The differentiation of the adipose-derived stem cells to parathyroid-like cells was confirmed by the detection of parathyroid hormone and the related parathyroid markers.
Results.
Adipose-derived stem cells were successfully isolated and purified from the rat adipocytes.
The adipogenic and osteogenic differentiation capabilities of the adipose-derived stem cells were determined.
SHH and activin A stimulated parathyroid hormone secretion by the adipose-derived stem cells and significantly increased the expression of calcium-sensing receptor (CaSR), parathyroid hormone, and glial cells missing homolog 2 (GCM2) in the cells in a time- and concentration-dependent manner.
Conclusion.
We successfully differentiated rat adipose-derived stem cells into parathyroid-like cells, which will pave a new route to curing hypoparathyroidism.
American Psychological Association (APA)
Zhang, Ping& Zhang, Hao& Dong, Wenwu& Wang, Zhihong& Qin, Yuan& Wu, Changhao…[et al.]. 2020. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
Modern Language Association (MLA)
Zhang, Ping…[et al.]. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
American Medical Association (AMA)
Zhang, Ping& Zhang, Hao& Dong, Wenwu& Wang, Zhihong& Qin, Yuan& Wu, Changhao…[et al.]. Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1170124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170124