TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion
المؤلفون المشاركون
Guan, Hongyu
Li, Yanbing
Yin, Yali
Huang, Yanrui
Chen, Shuwei
Zhang, Quan
Yu, Shuang
Hong, Shubin
Cao, Siting
Liu, Yujie
Xiao, Haipeng
المصدر
International Journal of Endocrinology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) promoting demethylation in cells.
However, the expression pattern and biologic significance of TET in papillary thyroid carcinoma (PTC) remain unclear.
This study aimed to elucidate the biological functions of TET1 and the miRNA and mRNA expression levels in PTC cells with downregulated TET1.
Methods.
The expression of the TET family in 49 PTC tissues and corresponding tumor-adjacent tissues, as well as PTC cell lines (BCPAP, K1, and TPC-1) and the normal thyroid epithelial cell line (Nthy-ori 3-1), were detected using quantitative real-time polymerase chain reaction.
The 5hmC level was detected in PTC tissues and cell lines using immunohistochemistry and dot blot assay, respectively.
After silencing the TET1 gene with siRNAs in BCPAP and TPC-1 cells, cell proliferation was detected using EdU assay.
Transwell assay was used to investigate cell migration and invasion.
miRNA and mRNA expression arrays were conducted in TET1-depleted BCPAP cells.
Results.
The expression level of TET1 decreased in PTC tissues and cell lines and was consistent with the reduction in the 5hmC level.
The knockdown of the TET1 gene promoted cell migration and invasion in BCPAP cells.
The expression of miR-7, miR-15/16 cluster, and let-7 family was downregulated, while the expression of let-7e was upregulated after siRNA-TET1 treatment in BCPAP cells.
The expression of WNT4, FZD4, CDK6, MCF2L, and EDN1 was upregulated as potential target genes of dysregulated miRNAs.
Conclusion.
The study showed that TET1 dysfunction inhibited the migration and invasion of BCPAP cells and might have a potential role in the pathogenesis of PTC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Shuang& Yin, Yali& Hong, Shubin& Cao, Siting& Huang, Yanrui& Chen, Shuwei…[et al.]. 2020. TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1170271
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Shuang…[et al.]. TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion. International Journal of Endocrinology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1170271
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Shuang& Yin, Yali& Hong, Shubin& Cao, Siting& Huang, Yanrui& Chen, Shuwei…[et al.]. TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1170271
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1170271
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر