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TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion
Joint Authors
Guan, Hongyu
Li, Yanbing
Yin, Yali
Huang, Yanrui
Chen, Shuwei
Zhang, Quan
Yu, Shuang
Hong, Shubin
Cao, Siting
Liu, Yujie
Xiao, Haipeng
Source
International Journal of Endocrinology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) promoting demethylation in cells.
However, the expression pattern and biologic significance of TET in papillary thyroid carcinoma (PTC) remain unclear.
This study aimed to elucidate the biological functions of TET1 and the miRNA and mRNA expression levels in PTC cells with downregulated TET1.
Methods.
The expression of the TET family in 49 PTC tissues and corresponding tumor-adjacent tissues, as well as PTC cell lines (BCPAP, K1, and TPC-1) and the normal thyroid epithelial cell line (Nthy-ori 3-1), were detected using quantitative real-time polymerase chain reaction.
The 5hmC level was detected in PTC tissues and cell lines using immunohistochemistry and dot blot assay, respectively.
After silencing the TET1 gene with siRNAs in BCPAP and TPC-1 cells, cell proliferation was detected using EdU assay.
Transwell assay was used to investigate cell migration and invasion.
miRNA and mRNA expression arrays were conducted in TET1-depleted BCPAP cells.
Results.
The expression level of TET1 decreased in PTC tissues and cell lines and was consistent with the reduction in the 5hmC level.
The knockdown of the TET1 gene promoted cell migration and invasion in BCPAP cells.
The expression of miR-7, miR-15/16 cluster, and let-7 family was downregulated, while the expression of let-7e was upregulated after siRNA-TET1 treatment in BCPAP cells.
The expression of WNT4, FZD4, CDK6, MCF2L, and EDN1 was upregulated as potential target genes of dysregulated miRNAs.
Conclusion.
The study showed that TET1 dysfunction inhibited the migration and invasion of BCPAP cells and might have a potential role in the pathogenesis of PTC.
American Psychological Association (APA)
Yu, Shuang& Yin, Yali& Hong, Shubin& Cao, Siting& Huang, Yanrui& Chen, Shuwei…[et al.]. 2020. TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion. International Journal of Endocrinology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1170271
Modern Language Association (MLA)
Yu, Shuang…[et al.]. TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion. International Journal of Endocrinology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1170271
American Medical Association (AMA)
Yu, Shuang& Yin, Yali& Hong, Shubin& Cao, Siting& Huang, Yanrui& Chen, Shuwei…[et al.]. TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion. International Journal of Endocrinology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1170271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170271