Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker

المؤلفون المشاركون

Xu, Yang
Wang, Shihan
Wang, Yanwei
Yang, Huailei
Lv, Zuying
Jin, Xiangqun
Wang, Yongsheng

المصدر

Journal of Analytical Methods in Chemistry

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-10-24

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الكيمياء

الملخص EN

A method, quantitative analysis of multicomponents by single marker (QAMS), was established in this article to investigate the quality control of a traditional Chinese medicine, Oviductus Ranae.

7-Hydroxycholesterol, 7-ketocholesterol, 4-cholesten-3-one, stigmasterol, 7-dehydrocholesterol, and cholesterol were selected as the indexes of quality evaluation of Oviductus Rana.

The determination was achieved on an Agilent HC-C18 column (4.6 mm × 250 mm, 5 μm) using methanol with water (87 : 13 v/v) as mobile phase at the flow rate of 2.0 mL/min.

Cholesterol was used as an internal standard to determine the relative correction factors between cholesterol and other steroidal constituents in Oviductus Ranae.

The contents of those steroidal constituents were calculated at the same time.

To evaluate the QAMS method, an external standard method was used to determine the contents of six steroidal constituents.

No significant difference was observed when comparing the quantitative results of QAMS with the results of external standard method.

The proposed QAMS method was proved to be accurate and feasible based on methodological experiments.

QAMS provided a simple, efficient, economical, and accurate way to control the quality of Oviductus Ranae.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Wang, Shihan& Xu, Yang& Wang, Yanwei& Yang, Huailei& Lv, Zuying& Jin, Xiangqun…[et al.]. 2017. Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170343

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Wang, Shihan…[et al.]. Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1170343

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Wang, Shihan& Xu, Yang& Wang, Yanwei& Yang, Huailei& Lv, Zuying& Jin, Xiangqun…[et al.]. Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170343

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1170343