Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker
Joint Authors
Xu, Yang
Wang, Shihan
Wang, Yanwei
Yang, Huailei
Lv, Zuying
Jin, Xiangqun
Wang, Yongsheng
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A method, quantitative analysis of multicomponents by single marker (QAMS), was established in this article to investigate the quality control of a traditional Chinese medicine, Oviductus Ranae.
7-Hydroxycholesterol, 7-ketocholesterol, 4-cholesten-3-one, stigmasterol, 7-dehydrocholesterol, and cholesterol were selected as the indexes of quality evaluation of Oviductus Rana.
The determination was achieved on an Agilent HC-C18 column (4.6 mm × 250 mm, 5 μm) using methanol with water (87 : 13 v/v) as mobile phase at the flow rate of 2.0 mL/min.
Cholesterol was used as an internal standard to determine the relative correction factors between cholesterol and other steroidal constituents in Oviductus Ranae.
The contents of those steroidal constituents were calculated at the same time.
To evaluate the QAMS method, an external standard method was used to determine the contents of six steroidal constituents.
No significant difference was observed when comparing the quantitative results of QAMS with the results of external standard method.
The proposed QAMS method was proved to be accurate and feasible based on methodological experiments.
QAMS provided a simple, efficient, economical, and accurate way to control the quality of Oviductus Ranae.
American Psychological Association (APA)
Wang, Shihan& Xu, Yang& Wang, Yanwei& Yang, Huailei& Lv, Zuying& Jin, Xiangqun…[et al.]. 2017. Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170343
Modern Language Association (MLA)
Wang, Shihan…[et al.]. Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1170343
American Medical Association (AMA)
Wang, Shihan& Xu, Yang& Wang, Yanwei& Yang, Huailei& Lv, Zuying& Jin, Xiangqun…[et al.]. Simultaneous Determination of Six Active Components in Oviductus Ranae via Quantitative Analysis of Multicomponents by Single Marker. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170343
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170343