Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing
المؤلفون المشاركون
Lu, Yanfang
Xu, Linfen
Huang, Lingna
Lian, Chengying
Xue, Huili
Chen, Xiujuan
Xia, Yong
المصدر
International Journal of Genomics
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-23
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women.
However, the detailed mechanism underlying embryonic miscarriage is not fully understood.
This study is aimed at analyzing embryonic miscarriage.
We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina.
Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of Lactobacillus, the major healthy microbe in the vagina.
Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined.
Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group.
This finding suggested that the immune response was suppressed in the embryonic miscarriage group.
To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed.
Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively.
At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group.
Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Linfen& Huang, Lingna& Lian, Chengying& Xue, Huili& Lu, Yanfang& Chen, Xiujuan…[et al.]. 2020. Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1171181
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Linfen…[et al.]. Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing. International Journal of Genomics No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1171181
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Linfen& Huang, Lingna& Lian, Chengying& Xue, Huili& Lu, Yanfang& Chen, Xiujuan…[et al.]. Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1171181
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171181
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر