Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing
Joint Authors
Lu, Yanfang
Xu, Linfen
Huang, Lingna
Lian, Chengying
Xue, Huili
Chen, Xiujuan
Xia, Yong
Source
International Journal of Genomics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women.
However, the detailed mechanism underlying embryonic miscarriage is not fully understood.
This study is aimed at analyzing embryonic miscarriage.
We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina.
Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of Lactobacillus, the major healthy microbe in the vagina.
Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined.
Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group.
This finding suggested that the immune response was suppressed in the embryonic miscarriage group.
To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed.
Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively.
At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group.
Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss.
American Psychological Association (APA)
Xu, Linfen& Huang, Lingna& Lian, Chengying& Xue, Huili& Lu, Yanfang& Chen, Xiujuan…[et al.]. 2020. Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1171181
Modern Language Association (MLA)
Xu, Linfen…[et al.]. Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing. International Journal of Genomics No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1171181
American Medical Association (AMA)
Xu, Linfen& Huang, Lingna& Lian, Chengying& Xue, Huili& Lu, Yanfang& Chen, Xiujuan…[et al.]. Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1171181
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171181