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Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil
المؤلفون المشاركون
Ehis-Eriakha, Chioma Bertha
Chikere, Chioma Blaise
Akaranta, Onyewuchi
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-30
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Crude oil pollution has consistently deteriorated all environmental compartments through the cycle of activities of the oil and gas industries.
However, there is a growing need to identify microbes with catabolic potentials to degrade these pollutants.
This research was conducted to identify bacteria with functional degradative genes.
A crude oil-polluted soil sample was obtained from an aged spill site at Imo River, Ebubu, Komkom community, Nigeria.
Bacteria isolates were obtained and screened for hydrocarbon degradation potential by turbidometry assay.
Plasmid and chromosomal DNA of the potential degraders were further screened for the presence of selected catabolic genes (C230, Alma, Alkb, nahAC, and PAHRHD(GP)) and identified by molecular typing.
Sixteen (16) out of the fifty (50) isolates obtained showed biodegradation activity in a liquid broth medium at varying levels.
Bacillus cereus showed highest potential for this assay with an optical density of 2.450 @ 600 nm wavelength.
Diverse catabolic genes resident in plasmids and chromosomes of the isolates and, in some cases, both plasmid and chromosomes of the same organism were observed.
The C230 gene was resident in >50% of the microbial population tested, while other genes occurred in lower proportions with the least observed in nahAC and PAHRHD.
These organisms can serve as potential bioremediation agents.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ehis-Eriakha, Chioma Bertha& Chikere, Chioma Blaise& Akaranta, Onyewuchi. 2020. Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172035
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ehis-Eriakha, Chioma Bertha…[et al.]. Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil. International Journal of Microbiology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1172035
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ehis-Eriakha, Chioma Bertha& Chikere, Chioma Blaise& Akaranta, Onyewuchi. Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172035
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172035
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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