Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil
Joint Authors
Ehis-Eriakha, Chioma Bertha
Chikere, Chioma Blaise
Akaranta, Onyewuchi
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Crude oil pollution has consistently deteriorated all environmental compartments through the cycle of activities of the oil and gas industries.
However, there is a growing need to identify microbes with catabolic potentials to degrade these pollutants.
This research was conducted to identify bacteria with functional degradative genes.
A crude oil-polluted soil sample was obtained from an aged spill site at Imo River, Ebubu, Komkom community, Nigeria.
Bacteria isolates were obtained and screened for hydrocarbon degradation potential by turbidometry assay.
Plasmid and chromosomal DNA of the potential degraders were further screened for the presence of selected catabolic genes (C230, Alma, Alkb, nahAC, and PAHRHD(GP)) and identified by molecular typing.
Sixteen (16) out of the fifty (50) isolates obtained showed biodegradation activity in a liquid broth medium at varying levels.
Bacillus cereus showed highest potential for this assay with an optical density of 2.450 @ 600 nm wavelength.
Diverse catabolic genes resident in plasmids and chromosomes of the isolates and, in some cases, both plasmid and chromosomes of the same organism were observed.
The C230 gene was resident in >50% of the microbial population tested, while other genes occurred in lower proportions with the least observed in nahAC and PAHRHD.
These organisms can serve as potential bioremediation agents.
American Psychological Association (APA)
Ehis-Eriakha, Chioma Bertha& Chikere, Chioma Blaise& Akaranta, Onyewuchi. 2020. Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172035
Modern Language Association (MLA)
Ehis-Eriakha, Chioma Bertha…[et al.]. Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil. International Journal of Microbiology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1172035
American Medical Association (AMA)
Ehis-Eriakha, Chioma Bertha& Chikere, Chioma Blaise& Akaranta, Onyewuchi. Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172035