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Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves
المؤلفون المشاركون
Alwash, Mourouge Saadi
Aqma, Wan Syaidatul
Ahmad, Wan Yaacob
Ibrahim, Nazlina
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-06
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Pseudomonas aeruginosa is one of the main causes of nosocomial infections and is frequently associated with opportunistic infections among hospitalized patients.
Kaempferol-3-O-(2′,6′-di-O-trans-p-coumaroyl)-β-D glucopyranoside (KF) is an antipseudomonal compound isolated from the leaves of the native medicinal plant Melastoma malabathricum.
Herein, an RNA-seq transcriptomic approach was employed to study the effect of KF treatment on P.
aeruginosa and to elucidate the molecular mechanisms underlying the response to KF at two time points (6 h and 24 h incubation).
Quantitative real-time PCR (qRT-PCR) was performed for four genes (uvrD, sodM, fumC1, and rpsL) to assess the reliability of the RNA-seq results.
The RNA-seq transcriptomic analysis revealed that KF increases the expression of genes involved in the electron transport chain (NADH-I), resulting in the induction of ATP synthesis.
Furthermore, KF also increased the expression of genes associated with ATP-binding cassette transporters, flagella, type III secretion system proteins, and DNA replication and repair, which may further influence nutrient uptake, motility, and growth.
The results also revealed that KF decreased the expression of a broad range of virulence factors associated with LPS biosynthesis, iron homeostasis, cytotoxic pigment pyocyanin production, and motility and adhesion that are representative of an acute P.
aeruginosa infection profile.
In addition, P.
aeruginosa pathways for amino acid synthesis and membrane lipid composition were modified to adapt to KF treatment.
Overall, the present research provides a detailed view of P.
aeruginosa adaptation and behaviour in response to KF and highlights the possible therapeutic approach of using plants to combat P.
aeruginosa infections.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alwash, Mourouge Saadi& Aqma, Wan Syaidatul& Ahmad, Wan Yaacob& Ibrahim, Nazlina. 2020. Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1172245
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alwash, Mourouge Saadi…[et al.]. Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves. International Journal of Microbiology No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1172245
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alwash, Mourouge Saadi& Aqma, Wan Syaidatul& Ahmad, Wan Yaacob& Ibrahim, Nazlina. Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1172245
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172245
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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