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Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves
Joint Authors
Alwash, Mourouge Saadi
Aqma, Wan Syaidatul
Ahmad, Wan Yaacob
Ibrahim, Nazlina
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-06
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Pseudomonas aeruginosa is one of the main causes of nosocomial infections and is frequently associated with opportunistic infections among hospitalized patients.
Kaempferol-3-O-(2′,6′-di-O-trans-p-coumaroyl)-β-D glucopyranoside (KF) is an antipseudomonal compound isolated from the leaves of the native medicinal plant Melastoma malabathricum.
Herein, an RNA-seq transcriptomic approach was employed to study the effect of KF treatment on P.
aeruginosa and to elucidate the molecular mechanisms underlying the response to KF at two time points (6 h and 24 h incubation).
Quantitative real-time PCR (qRT-PCR) was performed for four genes (uvrD, sodM, fumC1, and rpsL) to assess the reliability of the RNA-seq results.
The RNA-seq transcriptomic analysis revealed that KF increases the expression of genes involved in the electron transport chain (NADH-I), resulting in the induction of ATP synthesis.
Furthermore, KF also increased the expression of genes associated with ATP-binding cassette transporters, flagella, type III secretion system proteins, and DNA replication and repair, which may further influence nutrient uptake, motility, and growth.
The results also revealed that KF decreased the expression of a broad range of virulence factors associated with LPS biosynthesis, iron homeostasis, cytotoxic pigment pyocyanin production, and motility and adhesion that are representative of an acute P.
aeruginosa infection profile.
In addition, P.
aeruginosa pathways for amino acid synthesis and membrane lipid composition were modified to adapt to KF treatment.
Overall, the present research provides a detailed view of P.
aeruginosa adaptation and behaviour in response to KF and highlights the possible therapeutic approach of using plants to combat P.
aeruginosa infections.
American Psychological Association (APA)
Alwash, Mourouge Saadi& Aqma, Wan Syaidatul& Ahmad, Wan Yaacob& Ibrahim, Nazlina. 2020. Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1172245
Modern Language Association (MLA)
Alwash, Mourouge Saadi…[et al.]. Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves. International Journal of Microbiology No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1172245
American Medical Association (AMA)
Alwash, Mourouge Saadi& Aqma, Wan Syaidatul& Ahmad, Wan Yaacob& Ibrahim, Nazlina. Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1172245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172245