Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse

المؤلفون المشاركون

Lee, Jae-Hyung
Kim, Minjung
Yu, Youngseok
Koh, InSong
Moon, Ji-Hoi

المصدر

International Journal of Genomics

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-03-22

دولة النشر

مصر

عدد الصفحات

13

التخصصات الرئيسية

الأحياء

الملخص EN

Long noncoding RNAs (lncRNAs) are emerging as an important controller affecting metabolic tissue development, signaling, and function.

However, little is known about the function and profile of lncRNAs in osteoblastic differentiation in mice.

Here, we analyzed the RNA-sequencing (RNA-Seq) datasets obtained for 18 days in two-day intervals from neonatal mouse calvarial pre-osteoblast-like cells.

Over the course of osteoblast differentiation, 4058 mRNAs and 3948 lncRNAs were differentially expressed, and they were grouped into 12 clusters according to the expression pattern by fuzzy c-means clustering.

Using weighted gene coexpression network analysis, we identified 9 modules related to the early differentiation stage (days 2–8) and 7 modules related to the late differentiation stage (days 10–18).

Gene ontology and KEGG pathway enrichment analysis revealed that the mRNA and lncRNA upregulated in the late differentiation stage are highly associated with osteogenesis.

We also identified 72 mRNA and 89 lncRNAs as potential markers including several novel markers for osteoblast differentiation and activation.

Our findings provide a valuable resource for mouse lncRNA study and improves our understanding of the biology of osteoblastic differentiation in mice.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Kim, Minjung& Yu, Youngseok& Moon, Ji-Hoi& Koh, InSong& Lee, Jae-Hyung. 2018. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1172848

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Kim, Minjung…[et al.]. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse. International Journal of Genomics No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1172848

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Kim, Minjung& Yu, Youngseok& Moon, Ji-Hoi& Koh, InSong& Lee, Jae-Hyung. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1172848

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1172848