Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse
Joint Authors
Lee, Jae-Hyung
Kim, Minjung
Yu, Youngseok
Koh, InSong
Moon, Ji-Hoi
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Long noncoding RNAs (lncRNAs) are emerging as an important controller affecting metabolic tissue development, signaling, and function.
However, little is known about the function and profile of lncRNAs in osteoblastic differentiation in mice.
Here, we analyzed the RNA-sequencing (RNA-Seq) datasets obtained for 18 days in two-day intervals from neonatal mouse calvarial pre-osteoblast-like cells.
Over the course of osteoblast differentiation, 4058 mRNAs and 3948 lncRNAs were differentially expressed, and they were grouped into 12 clusters according to the expression pattern by fuzzy c-means clustering.
Using weighted gene coexpression network analysis, we identified 9 modules related to the early differentiation stage (days 2–8) and 7 modules related to the late differentiation stage (days 10–18).
Gene ontology and KEGG pathway enrichment analysis revealed that the mRNA and lncRNA upregulated in the late differentiation stage are highly associated with osteogenesis.
We also identified 72 mRNA and 89 lncRNAs as potential markers including several novel markers for osteoblast differentiation and activation.
Our findings provide a valuable resource for mouse lncRNA study and improves our understanding of the biology of osteoblastic differentiation in mice.
American Psychological Association (APA)
Kim, Minjung& Yu, Youngseok& Moon, Ji-Hoi& Koh, InSong& Lee, Jae-Hyung. 2018. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1172848
Modern Language Association (MLA)
Kim, Minjung…[et al.]. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse. International Journal of Genomics No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1172848
American Medical Association (AMA)
Kim, Minjung& Yu, Youngseok& Moon, Ji-Hoi& Koh, InSong& Lee, Jae-Hyung. Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1172848
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172848