Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli
المؤلفون المشاركون
Silva-Rocha, Rafael
Sanches-Medeiros, Ananda
Monteiro, Lummy Maria Oliveira
المصدر
International Journal of Genomics
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-21
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The engineering of synthetic circuits in cells relies on the use of well-characterized biological parts that would perform predicted functions under the situation considered, and many efforts have been taken to set biological standards that could define the basic features of these parts.
However, since most synthetic biology projects usually require a particular cellular chassis and set of growth conditions, defining standards in the field is not a simple task as gene expression measurements could be affected severely by genetic background and culture conditions.
In this study, we addressed promoter parameterization in bacteria in different genetic backgrounds and growth conditions.
We found that a small set of constitutive promoters of different strengths controlling a short-lived GFP reporter placed in a low-copy number plasmid produces remarkably reproducible results that allow for the calibration of promoter activity over different genetic backgrounds and physiological conditions, thus providing a simple way to set standards of promoter activity in bacteria.
Based on these results, we proposed the utilization of synthetic constitutive promoters as tools for calibration for the standardization of biological parts, in a way similar to the use of DNA and protein ladders in molecular biology as references for comparison with samples of interest.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sanches-Medeiros, Ananda& Monteiro, Lummy Maria Oliveira& Silva-Rocha, Rafael. 2018. Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172917
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sanches-Medeiros, Ananda…[et al.]. Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli. International Journal of Genomics No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1172917
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sanches-Medeiros, Ananda& Monteiro, Lummy Maria Oliveira& Silva-Rocha, Rafael. Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172917
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172917
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر