Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli
Joint Authors
Silva-Rocha, Rafael
Sanches-Medeiros, Ananda
Monteiro, Lummy Maria Oliveira
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The engineering of synthetic circuits in cells relies on the use of well-characterized biological parts that would perform predicted functions under the situation considered, and many efforts have been taken to set biological standards that could define the basic features of these parts.
However, since most synthetic biology projects usually require a particular cellular chassis and set of growth conditions, defining standards in the field is not a simple task as gene expression measurements could be affected severely by genetic background and culture conditions.
In this study, we addressed promoter parameterization in bacteria in different genetic backgrounds and growth conditions.
We found that a small set of constitutive promoters of different strengths controlling a short-lived GFP reporter placed in a low-copy number plasmid produces remarkably reproducible results that allow for the calibration of promoter activity over different genetic backgrounds and physiological conditions, thus providing a simple way to set standards of promoter activity in bacteria.
Based on these results, we proposed the utilization of synthetic constitutive promoters as tools for calibration for the standardization of biological parts, in a way similar to the use of DNA and protein ladders in molecular biology as references for comparison with samples of interest.
American Psychological Association (APA)
Sanches-Medeiros, Ananda& Monteiro, Lummy Maria Oliveira& Silva-Rocha, Rafael. 2018. Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172917
Modern Language Association (MLA)
Sanches-Medeiros, Ananda…[et al.]. Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli. International Journal of Genomics No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1172917
American Medical Association (AMA)
Sanches-Medeiros, Ananda& Monteiro, Lummy Maria Oliveira& Silva-Rocha, Rafael. Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators in Escherichia coli. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172917
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172917