Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling
المؤلفون المشاركون
Li, Yubin
Long, Lingli
Yu, Shuang
Li, Xiang
Hu, Yue
Long, Tengfei
Wang, Liqin
Li, Wenwen
Ye, Xiaoxin
Ke, Zunfu
Xiao, Haipeng
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-28
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Background.
Diabetic retinopathy (DR) is a serious microvascular complication of diabetes.
This study demonstrates the antiangiogenic effects of scutellarin (SCU) on high glucose- and hypoxia-stimulated human retinal endothelial cells (HRECs) and on a diabetic rat model by oral administration.
The antiangiogenic mechanisms of SCU in vitro and in vivo were investigated.
Method.
HRECs were cultured in high glucose- (30 mM D-glucose) and hypoxia (cobalt chloride-treated)-stimulated diabetic condition to evaluate the antiangiogenic effects of SCU by CCK-8 test, cell migration experiment (wound healing and transwell), and tube formation experiment.
A streptozotocin-induced type II diabetic rat model was established to measure the effects of oral administration of SCU on protecting retinal microvascular dysfunction by Doppler waveforms and HE staining.
We further used western blot, luciferase reporter assay, and immunofluorescence staining to study the antiangiogenic mechanism of SCU.
The protein levels of phospho-ERK, phospho-FAK, phospho-Src, VEGF, and PEDF were examined in HRECs and retina of diabetic rats.
Result.
Our results indicated that SCU attenuated diabetes-induced HREC proliferation, migration, and tube formation and decreased neovascularization and resistive index in the retina of diabetic rats by oral administration.
SCU suppressed the crosstalk of phospho-ERK, phospho-FAK, phospho-Src, and VEGF in vivo and in vitro.
Conclusions.
These results suggested that SCU can be an oral drug to alleviate microvascular dysfunction of DR and exerts its antiangiogenic effects by inhibiting the expression of the crosstalk of VEGF, p-ERK, p-FAK, and p-Src.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Long, Lingli& Li, Yubin& Yu, Shuang& Li, Xiang& Hu, Yue& Long, Tengfei…[et al.]. 2019. Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1172973
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Long, Lingli…[et al.]. Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling. Journal of Diabetes Research No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1172973
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Long, Lingli& Li, Yubin& Yu, Shuang& Li, Xiang& Hu, Yue& Long, Tengfei…[et al.]. Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1172973
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172973
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر