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Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling
Joint Authors
Li, Yubin
Long, Lingli
Yu, Shuang
Li, Xiang
Hu, Yue
Long, Tengfei
Wang, Liqin
Li, Wenwen
Ye, Xiaoxin
Ke, Zunfu
Xiao, Haipeng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-28
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Background.
Diabetic retinopathy (DR) is a serious microvascular complication of diabetes.
This study demonstrates the antiangiogenic effects of scutellarin (SCU) on high glucose- and hypoxia-stimulated human retinal endothelial cells (HRECs) and on a diabetic rat model by oral administration.
The antiangiogenic mechanisms of SCU in vitro and in vivo were investigated.
Method.
HRECs were cultured in high glucose- (30 mM D-glucose) and hypoxia (cobalt chloride-treated)-stimulated diabetic condition to evaluate the antiangiogenic effects of SCU by CCK-8 test, cell migration experiment (wound healing and transwell), and tube formation experiment.
A streptozotocin-induced type II diabetic rat model was established to measure the effects of oral administration of SCU on protecting retinal microvascular dysfunction by Doppler waveforms and HE staining.
We further used western blot, luciferase reporter assay, and immunofluorescence staining to study the antiangiogenic mechanism of SCU.
The protein levels of phospho-ERK, phospho-FAK, phospho-Src, VEGF, and PEDF were examined in HRECs and retina of diabetic rats.
Result.
Our results indicated that SCU attenuated diabetes-induced HREC proliferation, migration, and tube formation and decreased neovascularization and resistive index in the retina of diabetic rats by oral administration.
SCU suppressed the crosstalk of phospho-ERK, phospho-FAK, phospho-Src, and VEGF in vivo and in vitro.
Conclusions.
These results suggested that SCU can be an oral drug to alleviate microvascular dysfunction of DR and exerts its antiangiogenic effects by inhibiting the expression of the crosstalk of VEGF, p-ERK, p-FAK, and p-Src.
American Psychological Association (APA)
Long, Lingli& Li, Yubin& Yu, Shuang& Li, Xiang& Hu, Yue& Long, Tengfei…[et al.]. 2019. Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1172973
Modern Language Association (MLA)
Long, Lingli…[et al.]. Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling. Journal of Diabetes Research No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1172973
American Medical Association (AMA)
Long, Lingli& Li, Yubin& Yu, Shuang& Li, Xiang& Hu, Yue& Long, Tengfei…[et al.]. Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGFERKFAKSrc Pathway Signaling. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1172973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172973