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Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya
المؤلفون المشاركون
Ndahebwa Muhonja, Christabel
Magoma, Gabriel
Imbuga, Mabel
Makonde, Huxley Mae
المصدر
International Journal of Microbiology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-12-03
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi.
Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively.
The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively.
Isolates were screened for their ability to produce extracellular laccase and esterase; Aspergillus fumigatus strain B2,2 exhibited the highest presence of laccase (15.67 mm) while Aspergillus oryzae strain A5,1 exhibited the highest presence of esterase (14.33 mm).
Alkane hydroxylase-encoding genes were screened for using primer AlkB 1 which amplified the fragment of size 870 bp.
Four bacterial samples were positive for the gene.
Optimum growth temperature of the fungal isolates was 30°C.
The possession of laccase, esterase, and alkane hydroxylase activities is suggested as key molecular basis for LDPE degrading capacity.
Knowledge of optimum growth conditions will serve to better utilize microbes in the bioremediation of LDPE.
The application of Aspergillus oryzae strain A5,1 and Bacillus cereus strain A5,a in polyethene degradation is a promising option in this kind of bioremediation as they exhibited significantly high levels of biodegradation.
Further investigation of more alkane degrading genes in biodegrading microbes will inform the choice of the right microbial consortia for bioaugmentation strategies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ndahebwa Muhonja, Christabel& Magoma, Gabriel& Imbuga, Mabel& Makonde, Huxley Mae. 2018. Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya. International Journal of Microbiology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1173321
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ndahebwa Muhonja, Christabel…[et al.]. Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya. International Journal of Microbiology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1173321
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ndahebwa Muhonja, Christabel& Magoma, Gabriel& Imbuga, Mabel& Makonde, Huxley Mae. Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya. International Journal of Microbiology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1173321
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1173321
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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