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Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya
Joint Authors
Ndahebwa Muhonja, Christabel
Magoma, Gabriel
Imbuga, Mabel
Makonde, Huxley Mae
Source
International Journal of Microbiology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi.
Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively.
The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively.
Isolates were screened for their ability to produce extracellular laccase and esterase; Aspergillus fumigatus strain B2,2 exhibited the highest presence of laccase (15.67 mm) while Aspergillus oryzae strain A5,1 exhibited the highest presence of esterase (14.33 mm).
Alkane hydroxylase-encoding genes were screened for using primer AlkB 1 which amplified the fragment of size 870 bp.
Four bacterial samples were positive for the gene.
Optimum growth temperature of the fungal isolates was 30°C.
The possession of laccase, esterase, and alkane hydroxylase activities is suggested as key molecular basis for LDPE degrading capacity.
Knowledge of optimum growth conditions will serve to better utilize microbes in the bioremediation of LDPE.
The application of Aspergillus oryzae strain A5,1 and Bacillus cereus strain A5,a in polyethene degradation is a promising option in this kind of bioremediation as they exhibited significantly high levels of biodegradation.
Further investigation of more alkane degrading genes in biodegrading microbes will inform the choice of the right microbial consortia for bioaugmentation strategies.
American Psychological Association (APA)
Ndahebwa Muhonja, Christabel& Magoma, Gabriel& Imbuga, Mabel& Makonde, Huxley Mae. 2018. Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya. International Journal of Microbiology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1173321
Modern Language Association (MLA)
Ndahebwa Muhonja, Christabel…[et al.]. Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya. International Journal of Microbiology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1173321
American Medical Association (AMA)
Ndahebwa Muhonja, Christabel& Magoma, Gabriel& Imbuga, Mabel& Makonde, Huxley Mae. Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya. International Journal of Microbiology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1173321
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173321