Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana
المؤلفون المشاركون
Owiredu, Eddie-Williams
Sakyi, Samuel Asamoah
Buckman, Tonnies Abeku
Antwi-Berko, Daniel
Yeboah-Mensah, Kwame
Dey, Dzifa
Amoani, Benjamin
Mantey, Richard
المصدر
International Journal of Rheumatology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
T cell cytokines play important roles in the development and progression of rheumatoid arthritis (RA).
Loss of Th1/Th2 and Th17/Treg balance has been reported in several inflammatory autoimmune diseases.
However, their role in RA within hitherto rare Ghanaian context has not been explored.
Here, we evaluated the intracytoplasmic CD4+ T cell cytokine patterns in rheumatoid arthritis patients in Ghana and determined their relationship with disease activity.
Methods.
This case-control study included 48 newly diagnosed RA patients and 30 apparent healthy controls from two major hospitals in Ghana.
Validated structured questionnaires were administered to obtain demographic data; blood samples were collected and processed for flow cytometric analysis.
Results.
IFN-γ, TNF-α, IL-4, IL-6, IL-10, IL-17A, IL-6/IL-4, and IL-17/IL-10 expressions were significantly higher in RA cases compared to the healthy controls.
The expression of IL-6 (0.00 (0.00-0.98) vs.
0.82 (0.34-1.10) vs.
1.56 (1.39-1.68), p<0.0001), IL-17A (0.00 (0.00-0.02) vs.
0.19 (0.09-0.30) vs.
0.99 (0.64-1.25), p<0.0001), and IL-17A/IL-10 (0.00 (0.00-0.39) vs.
0.15 (0.09-0.26) vs.
0.88 (0.41-1.47), p<0.0001) increased significantly from the healthy controls through RA patients with low DAS scores to RA patients with moderate DAS scores.
IL-6 (β=0.681, r2=0.527, p<0.0001), IL-17A (β=0.770, r2=0.593, p<0.0001), and IL-17A/IL-10 (β=0.677, r2=0.452, p<0.0001) expressions were significantly directly associated with DAS28 scores.
IL-6 (cutoff=1.32, sensitivity=100.0%, specificity=100.0%, accuracy=100.0%, and AUC=1.000) and IL-17A (cutoff=0.58, sensitivity=100.0%, specificity=100.0%, accuracy=100.0%, and AUC=1.000) presented with the best discriminatory power in predicting moderate DAS scores from low DAS scores.
Conclusion.
Th1- and Th17-related cytokines predominate in the pathophysiology of RA, with IL-6 and IL-17 being principally and differentially expressed based on the severity of the disease.
IL-6 and IL-17A could serve as useful prognostic and disease-monitoring markers in RA in the African context.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sakyi, Samuel Asamoah& Buckman, Tonnies Abeku& Antwi-Berko, Daniel& Yeboah-Mensah, Kwame& Dey, Dzifa& Owiredu, Eddie-Williams…[et al.]. 2020. Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana. International Journal of Rheumatology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1173974
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sakyi, Samuel Asamoah…[et al.]. Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana. International Journal of Rheumatology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1173974
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sakyi, Samuel Asamoah& Buckman, Tonnies Abeku& Antwi-Berko, Daniel& Yeboah-Mensah, Kwame& Dey, Dzifa& Owiredu, Eddie-Williams…[et al.]. Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana. International Journal of Rheumatology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1173974
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1173974
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر