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Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana
Joint Authors
Owiredu, Eddie-Williams
Sakyi, Samuel Asamoah
Buckman, Tonnies Abeku
Antwi-Berko, Daniel
Yeboah-Mensah, Kwame
Dey, Dzifa
Amoani, Benjamin
Mantey, Richard
Source
International Journal of Rheumatology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
T cell cytokines play important roles in the development and progression of rheumatoid arthritis (RA).
Loss of Th1/Th2 and Th17/Treg balance has been reported in several inflammatory autoimmune diseases.
However, their role in RA within hitherto rare Ghanaian context has not been explored.
Here, we evaluated the intracytoplasmic CD4+ T cell cytokine patterns in rheumatoid arthritis patients in Ghana and determined their relationship with disease activity.
Methods.
This case-control study included 48 newly diagnosed RA patients and 30 apparent healthy controls from two major hospitals in Ghana.
Validated structured questionnaires were administered to obtain demographic data; blood samples were collected and processed for flow cytometric analysis.
Results.
IFN-γ, TNF-α, IL-4, IL-6, IL-10, IL-17A, IL-6/IL-4, and IL-17/IL-10 expressions were significantly higher in RA cases compared to the healthy controls.
The expression of IL-6 (0.00 (0.00-0.98) vs.
0.82 (0.34-1.10) vs.
1.56 (1.39-1.68), p<0.0001), IL-17A (0.00 (0.00-0.02) vs.
0.19 (0.09-0.30) vs.
0.99 (0.64-1.25), p<0.0001), and IL-17A/IL-10 (0.00 (0.00-0.39) vs.
0.15 (0.09-0.26) vs.
0.88 (0.41-1.47), p<0.0001) increased significantly from the healthy controls through RA patients with low DAS scores to RA patients with moderate DAS scores.
IL-6 (β=0.681, r2=0.527, p<0.0001), IL-17A (β=0.770, r2=0.593, p<0.0001), and IL-17A/IL-10 (β=0.677, r2=0.452, p<0.0001) expressions were significantly directly associated with DAS28 scores.
IL-6 (cutoff=1.32, sensitivity=100.0%, specificity=100.0%, accuracy=100.0%, and AUC=1.000) and IL-17A (cutoff=0.58, sensitivity=100.0%, specificity=100.0%, accuracy=100.0%, and AUC=1.000) presented with the best discriminatory power in predicting moderate DAS scores from low DAS scores.
Conclusion.
Th1- and Th17-related cytokines predominate in the pathophysiology of RA, with IL-6 and IL-17 being principally and differentially expressed based on the severity of the disease.
IL-6 and IL-17A could serve as useful prognostic and disease-monitoring markers in RA in the African context.
American Psychological Association (APA)
Sakyi, Samuel Asamoah& Buckman, Tonnies Abeku& Antwi-Berko, Daniel& Yeboah-Mensah, Kwame& Dey, Dzifa& Owiredu, Eddie-Williams…[et al.]. 2020. Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana. International Journal of Rheumatology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1173974
Modern Language Association (MLA)
Sakyi, Samuel Asamoah…[et al.]. Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana. International Journal of Rheumatology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1173974
American Medical Association (AMA)
Sakyi, Samuel Asamoah& Buckman, Tonnies Abeku& Antwi-Berko, Daniel& Yeboah-Mensah, Kwame& Dey, Dzifa& Owiredu, Eddie-Williams…[et al.]. Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana. International Journal of Rheumatology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1173974
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173974