Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method
المؤلفون المشاركون
Li, Mingming
Gao, Shouhong
Chen, Wansheng
Wang, Zhipeng
Wei, Hua
Zhong, Renqian
Zhang, Feng
Yang, Yang
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-01
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
A targeted ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the simultaneous determination of 34 amino acids in tissue samples from colorectal cancer (CRC) patients.
The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column (3.0 × 150 mm, 5 μm) with a binary gradient elution system (A, 0.02% heptafluorobutyric acid and 0.2% formic acid in water, v/v; B, methanol).
The run time was 10 min.
The multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition.
The linear correlation coefficients were >0.99 for all the analytes in their corresponding calibration ranges.
The sample was pretreated based on tissue homogenate and protein precipitation with a 100 mg aliquot sample.
The average recovery and matrix effect for 34 amino acids and 3 internal standards were 39.00%∼146.95% and 49.45%∼173.63%, respectively.
The intra- and interday accuracy for all the analytes ranged from −13.52% to 14.21% (RSD ≤8.57%) and from −14.52% to 12.59% (RSD ≤10.31%), respectively.
Deviations of stability under different conditions were within ±15% for all the analytes.
This method was applied to simultaneous quantification of 34 amino acids in tissue samples from 94 CRC patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Yang& Zhang, Feng& Gao, Shouhong& Wang, Zhipeng& Li, Mingming& Wei, Hua…[et al.]. 2020. Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1174749
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Yang…[et al.]. Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1174749
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Yang& Zhang, Feng& Gao, Shouhong& Wang, Zhipeng& Li, Mingming& Wei, Hua…[et al.]. Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1174749
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1174749
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر