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Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats
المؤلفون المشاركون
Li, Xiaoxin
He, Aiping
Zhou, Chunling
Tian, Jinmiao
Jiang, Meihua
Fang, Lina
Liu, Tianzhu
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-17
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
VS-5584 is a small-molecular compound that showed equivalent activity against mTOR and all class I PI3K isoforms and demonstrated preclinical activity in diverse cancer cell lines and xenograft tumor model, and rational combination of VS-5584 and other target therapies achieved promising outcomes in oncology.
In the present study, we established and validated a simple and sensitive UPLC-MS/MS method for the determination of VS-5584 in plasma samples.
VS-5584 was separated via an Acquity UPLC BEH C18 column, with a mobile phase composed of acetonitrile and 0.2% formic acid in water (40 : 60).
The calibration curve displayed a good linearity in the range of 1.0–1000 ng/mL, with satisfactory accuracy (−13.6% < RE% < 8.8%) and precision (CV%, less than 9.2%).
The validated method was then applied to a pharmacokinetic study in rats.
After administration of 10 mg/kg, VS-5584 was absorbed quickly and reached a peak concentration of 473.2 ± 72.0 ng/mL after 20 min.
The established method allows for the quantification of VS-5584 in rat plasma in detail and can be utilized to successfully describe the pharmacokinetic profile of VS-5584.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Chunling& Li, Xiaoxin& He, Aiping& Liu, Tianzhu& Tian, Jinmiao& Jiang, Meihua…[et al.]. 2020. Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1174892
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Chunling…[et al.]. Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1174892
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Chunling& Li, Xiaoxin& He, Aiping& Liu, Tianzhu& Tian, Jinmiao& Jiang, Meihua…[et al.]. Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1174892
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1174892
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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