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Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum
المؤلفون المشاركون
Zheng, Yunliang
Xu, Nana
Zhang, Qiao
Liu, Yanpeng
Zhao, Qingwei
Hu, Xingjiang
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample.
Samples were pretreated by a single-step precipitation with acetonitrile.
Chromatographic separation was achieved on XSelecT HSS T3 column by gradient elution with a total run time of 5.0 min.
MS detection was performed by a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode with a positive electrospray ionization source.
Isotope-labeled internal standard, especially rifampicin-D8, was applied to adjust for the loss during sample treatment.
The established LC-MS/MS method showed a wide analytical range (pyrazinamide: 1.02∼60.0 μg/mL, isoniazid: 0.152∼10.0 μg/mL, rifampicin: 0.500∼30.0 μg/mL, and ethambutol: 0.0998∼5.99 μg/mL) and a good linearity (r > 0.99 for the four analytes) with acceptable accuracy and precision (90.15%∼104.62% and 94.00%∼104.02% for intra- and interaccuracy, respectively; RSD%: <12.46% and <6.43% for intra- and interprecision, respectively).
It also showed excellent recoveries (79.24%∼94.16% for all analytes) and absence of significant matrix effect.
This method was successfully applied to the quantification of four first-line antituberculosis (anti-TB) drugs, suggesting its suitability for therapeutic drug monitoring in the clinical practices.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zheng, Yunliang& Xu, Nana& Hu, Xingjiang& Zhang, Qiao& Liu, Yanpeng& Zhao, Qingwei. 2020. Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1174928
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zheng, Yunliang…[et al.]. Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1174928
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zheng, Yunliang& Xu, Nana& Hu, Xingjiang& Zhang, Qiao& Liu, Yanpeng& Zhao, Qingwei. Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1174928
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1174928
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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