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Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum
Joint Authors
Zheng, Yunliang
Xu, Nana
Zhang, Qiao
Liu, Yanpeng
Zhao, Qingwei
Hu, Xingjiang
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample.
Samples were pretreated by a single-step precipitation with acetonitrile.
Chromatographic separation was achieved on XSelecT HSS T3 column by gradient elution with a total run time of 5.0 min.
MS detection was performed by a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode with a positive electrospray ionization source.
Isotope-labeled internal standard, especially rifampicin-D8, was applied to adjust for the loss during sample treatment.
The established LC-MS/MS method showed a wide analytical range (pyrazinamide: 1.02∼60.0 μg/mL, isoniazid: 0.152∼10.0 μg/mL, rifampicin: 0.500∼30.0 μg/mL, and ethambutol: 0.0998∼5.99 μg/mL) and a good linearity (r > 0.99 for the four analytes) with acceptable accuracy and precision (90.15%∼104.62% and 94.00%∼104.02% for intra- and interaccuracy, respectively; RSD%: <12.46% and <6.43% for intra- and interprecision, respectively).
It also showed excellent recoveries (79.24%∼94.16% for all analytes) and absence of significant matrix effect.
This method was successfully applied to the quantification of four first-line antituberculosis (anti-TB) drugs, suggesting its suitability for therapeutic drug monitoring in the clinical practices.
American Psychological Association (APA)
Zheng, Yunliang& Xu, Nana& Hu, Xingjiang& Zhang, Qiao& Liu, Yanpeng& Zhao, Qingwei. 2020. Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1174928
Modern Language Association (MLA)
Zheng, Yunliang…[et al.]. Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1174928
American Medical Association (AMA)
Zheng, Yunliang& Xu, Nana& Hu, Xingjiang& Zhang, Qiao& Liu, Yanpeng& Zhao, Qingwei. Development and Application of a LC-MSMS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1174928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174928