Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus
المؤلفون المشاركون
Abuaf, Nisen
Desgruelles, Chantal
Moumaris, Mohamed
Boussa-Khettab, Faïza
Rostane, Hidayeth
Bellec, Emilie
Frances, Camille
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-05
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
A new method for the detection by flow cytometry of anti-double-stranded DNA antibodies and of circulating immune complexes (IC) containing endogenous DNA (IC-eDNA) is described.
From each serum sample, two samples were taken, one was used to detect IC-eDNA.
The other to detect anti-DNA antibodies was incubated with calf thymus DNA.
ICs were isolated by polyethylene glycol precipitation or by cryoprecipitation, after which immunoglobulins were labeled with FITC-conjugated anti-human globulin.
Serum samples from 63 systemic lupus erythematosus (SLE) patients, 32 incomplete lupus, and 87 control patients were tested.
Detection of anti-dsDNA antibodies by flow cytometry had a diagnostic sensitivity and specificity almost comparable to routine tests, the fluorescent enzyme immunoassay EliA™-dsDNA test, and the ultrasensitive Crithidia luciliae indirect immunofluorescence test.
In 21 (33%) out of 63 SLE serum samples, IC-eDNA was detected.
In these samples, free anti-dsDNA antibodies were hardly detectable or undetectable by flow cytometry or by routine tests.
When anti-DNA antibodies are neutralized by endogenous DNA and can no longer be detected by routine tests, the serologic diagnosis and the follow-up of relapses in patients with SLE is compromised.
To overcome this obstacle, we propose an accessible solution: the detection of circulating IC-eDNA by flow cytometry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abuaf, Nisen& Desgruelles, Chantal& Moumaris, Mohamed& Boussa-Khettab, Faïza& Rostane, Hidayeth& Bellec, Emilie…[et al.]. 2019. Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176408
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abuaf, Nisen…[et al.]. Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus. Journal of Immunology Research No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1176408
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abuaf, Nisen& Desgruelles, Chantal& Moumaris, Mohamed& Boussa-Khettab, Faïza& Rostane, Hidayeth& Bellec, Emilie…[et al.]. Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176408
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176408
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر