Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus
Joint Authors
Abuaf, Nisen
Desgruelles, Chantal
Moumaris, Mohamed
Boussa-Khettab, Faïza
Rostane, Hidayeth
Bellec, Emilie
Frances, Camille
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A new method for the detection by flow cytometry of anti-double-stranded DNA antibodies and of circulating immune complexes (IC) containing endogenous DNA (IC-eDNA) is described.
From each serum sample, two samples were taken, one was used to detect IC-eDNA.
The other to detect anti-DNA antibodies was incubated with calf thymus DNA.
ICs were isolated by polyethylene glycol precipitation or by cryoprecipitation, after which immunoglobulins were labeled with FITC-conjugated anti-human globulin.
Serum samples from 63 systemic lupus erythematosus (SLE) patients, 32 incomplete lupus, and 87 control patients were tested.
Detection of anti-dsDNA antibodies by flow cytometry had a diagnostic sensitivity and specificity almost comparable to routine tests, the fluorescent enzyme immunoassay EliA™-dsDNA test, and the ultrasensitive Crithidia luciliae indirect immunofluorescence test.
In 21 (33%) out of 63 SLE serum samples, IC-eDNA was detected.
In these samples, free anti-dsDNA antibodies were hardly detectable or undetectable by flow cytometry or by routine tests.
When anti-DNA antibodies are neutralized by endogenous DNA and can no longer be detected by routine tests, the serologic diagnosis and the follow-up of relapses in patients with SLE is compromised.
To overcome this obstacle, we propose an accessible solution: the detection of circulating IC-eDNA by flow cytometry.
American Psychological Association (APA)
Abuaf, Nisen& Desgruelles, Chantal& Moumaris, Mohamed& Boussa-Khettab, Faïza& Rostane, Hidayeth& Bellec, Emilie…[et al.]. 2019. Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176408
Modern Language Association (MLA)
Abuaf, Nisen…[et al.]. Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus. Journal of Immunology Research No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1176408
American Medical Association (AMA)
Abuaf, Nisen& Desgruelles, Chantal& Moumaris, Mohamed& Boussa-Khettab, Faïza& Rostane, Hidayeth& Bellec, Emilie…[et al.]. Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176408