Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

المؤلفون المشاركون

Le, Hoa Thi
Park, Kyu H.
Jung, Woong
Park, Hyung Soon
Kim, Tae Woo

المصدر

Journal of Analytical Methods in Chemistry

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-10-21

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الكيمياء

الملخص EN

We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum.

The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS.

The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000 m/z range.

Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans.

However, with the established method, i.e., DHB/An method, only 15 glycans were assigned.

To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Le, Hoa Thi& Park, Kyu H.& Jung, Woong& Park, Hyung Soon& Kim, Tae Woo. 2018. Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176533

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Le, Hoa Thi…[et al.]. Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176533

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Le, Hoa Thi& Park, Kyu H.& Jung, Woong& Park, Hyung Soon& Kim, Tae Woo. Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176533

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1176533