Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans
Joint Authors
Le, Hoa Thi
Park, Kyu H.
Jung, Woong
Park, Hyung Soon
Kim, Tae Woo
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum.
The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS.
The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000 m/z range.
Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans.
However, with the established method, i.e., DHB/An method, only 15 glycans were assigned.
To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.
American Psychological Association (APA)
Le, Hoa Thi& Park, Kyu H.& Jung, Woong& Park, Hyung Soon& Kim, Tae Woo. 2018. Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176533
Modern Language Association (MLA)
Le, Hoa Thi…[et al.]. Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176533
American Medical Association (AMA)
Le, Hoa Thi& Park, Kyu H.& Jung, Woong& Park, Hyung Soon& Kim, Tae Woo. Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176533
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176533