A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor
المؤلفون المشاركون
Kim, Hye Jin
Kang, Dong-Hoon
Yang, Seung-Hoon
Lee, Eunji
Ha, Taewon
Lee, Byung Chul
Kim, Youngbaek
Hwang, Kyo Seon
Shin, Hyun-Joon
Kim, Jinsik
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-11
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Dielectrophoresis- (DEP-) based separation method between a protein, amyloid beta 42, and polystyrene (PS) beads in different microholes was demonstrated for enhancement of performance for bead-based fluorescent sensor.
An intensity of ∇E2 was relative to a diameter of a microhole, and the diameters of two microholes for separation between the protein and PS beads were simulated to 3 μm and 15 μm, respectively.
The microholes were fabricated by microelectromechanical systems (MEMS).
The separation between the protein and the PS beads was demonstrated by comparing the average intensity of fluorescence (AIF) by each molecule.
Relative AIF was measured in various applying voltage and time conditions, and the conditions for allocating the PS beads into 15 μm hole were optimized at 80 mV and 15 min, respectively.
In the optimized condition, the relative AIF was observed approximately 4.908 ± 0.299.
Finally, in 3 μm and 15 μm hole, the AIFs were approximately 3.143 and −1.346 by 2 nm of protein and about −2.515 and 4.211 by 30 nm of the PS beads, respectively.
The results showed that 2 nm of the protein and 30 nm of PS beads were separated by DEP force in each microhole effectively, and that our method is applicable as a new method to verify an efficiency of the labeling for bead-based fluorescent sensor ∇E2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Hye Jin& Kang, Dong-Hoon& Yang, Seung-Hoon& Lee, Eunji& Ha, Taewon& Lee, Byung Chul…[et al.]. 2018. A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176600
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Hye Jin…[et al.]. A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176600
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Hye Jin& Kang, Dong-Hoon& Yang, Seung-Hoon& Lee, Eunji& Ha, Taewon& Lee, Byung Chul…[et al.]. A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176600
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176600
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر