A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor
Joint Authors
Kim, Hye Jin
Kang, Dong-Hoon
Yang, Seung-Hoon
Lee, Eunji
Ha, Taewon
Lee, Byung Chul
Kim, Youngbaek
Hwang, Kyo Seon
Shin, Hyun-Joon
Kim, Jinsik
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Dielectrophoresis- (DEP-) based separation method between a protein, amyloid beta 42, and polystyrene (PS) beads in different microholes was demonstrated for enhancement of performance for bead-based fluorescent sensor.
An intensity of ∇E2 was relative to a diameter of a microhole, and the diameters of two microholes for separation between the protein and PS beads were simulated to 3 μm and 15 μm, respectively.
The microholes were fabricated by microelectromechanical systems (MEMS).
The separation between the protein and the PS beads was demonstrated by comparing the average intensity of fluorescence (AIF) by each molecule.
Relative AIF was measured in various applying voltage and time conditions, and the conditions for allocating the PS beads into 15 μm hole were optimized at 80 mV and 15 min, respectively.
In the optimized condition, the relative AIF was observed approximately 4.908 ± 0.299.
Finally, in 3 μm and 15 μm hole, the AIFs were approximately 3.143 and −1.346 by 2 nm of protein and about −2.515 and 4.211 by 30 nm of the PS beads, respectively.
The results showed that 2 nm of the protein and 30 nm of PS beads were separated by DEP force in each microhole effectively, and that our method is applicable as a new method to verify an efficiency of the labeling for bead-based fluorescent sensor ∇E2.
American Psychological Association (APA)
Kim, Hye Jin& Kang, Dong-Hoon& Yang, Seung-Hoon& Lee, Eunji& Ha, Taewon& Lee, Byung Chul…[et al.]. 2018. A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176600
Modern Language Association (MLA)
Kim, Hye Jin…[et al.]. A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176600
American Medical Association (AMA)
Kim, Hye Jin& Kang, Dong-Hoon& Yang, Seung-Hoon& Lee, Eunji& Ha, Taewon& Lee, Byung Chul…[et al.]. A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176600