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The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice
المؤلفون المشاركون
Sennikov, Sergey V.
Kurilin, Vasiliy V.
Khantakova, Julia N.
Tereschenko, Valeriy P.
Obleukhova, Irina A.
Anatolievna, Lopatnikova Julia
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-18
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Introduction.
Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses.
In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice.
Methods.
DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4.
DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes.
The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated.
Results.
Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice.
In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86).
Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules.
Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10.
In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production.
Conclusion.
These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments.
In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kurilin, Vasiliy V.& Khantakova, Julia N.& Tereschenko, Valeriy P.& Anatolievna, Lopatnikova Julia& Obleukhova, Irina A.& Sennikov, Sergey V.. 2019. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176626
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kurilin, Vasiliy V.…[et al.]. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice. Journal of Immunology Research No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1176626
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kurilin, Vasiliy V.& Khantakova, Julia N.& Tereschenko, Valeriy P.& Anatolievna, Lopatnikova Julia& Obleukhova, Irina A.& Sennikov, Sergey V.. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176626
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176626
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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