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The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice
Joint Authors
Sennikov, Sergey V.
Kurilin, Vasiliy V.
Khantakova, Julia N.
Tereschenko, Valeriy P.
Obleukhova, Irina A.
Anatolievna, Lopatnikova Julia
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Introduction.
Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses.
In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice.
Methods.
DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4.
DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes.
The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated.
Results.
Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice.
In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86).
Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules.
Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10.
In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production.
Conclusion.
These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments.
In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.
American Psychological Association (APA)
Kurilin, Vasiliy V.& Khantakova, Julia N.& Tereschenko, Valeriy P.& Anatolievna, Lopatnikova Julia& Obleukhova, Irina A.& Sennikov, Sergey V.. 2019. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176626
Modern Language Association (MLA)
Kurilin, Vasiliy V.…[et al.]. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice. Journal of Immunology Research No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1176626
American Medical Association (AMA)
Kurilin, Vasiliy V.& Khantakova, Julia N.& Tereschenko, Valeriy P.& Anatolievna, Lopatnikova Julia& Obleukhova, Irina A.& Sennikov, Sergey V.. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL6 and CBA Mice. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176626