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Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS
المؤلفون المشاركون
Liu, Jinlai
Weng, Qinghua
Li, Feifei
Zhang, Xueliang
Zhou, Yunfang
Chen, Lian-Guo
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-05
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The aim of this study is to establish and validate a rapid, selective, and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine tubeimoside I (TBMS-I) in ICR (Institute of Cancer Research) mouse whole blood and its application in the pharmacokinetics and bioavailability study.
The blood samples were precipitated by acetonitrile to extract the analytes.
Chromatographic separation was performed on a UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm).
The mobile phase consisted of water with 0.1% formic acid and methanol (1 : 1, v/v) at a flow rate of 0.4 mL/min.
The total eluting time was 4 min.
The TBMS-I and ardisiacrispin A (internal standard (IS)) were quantitatively detected by a tandem mass spectrometry equipped with an electrospray ionization (ESI) in a positive mode by multiple reaction monitoring (MRM).
A validation of this method was in accordance with the US Food and Drug Administration (FDA) guidelines.
The lower limit of quantification (LLOQ) of TBMS-I was 2 ng/mL, and the calibration curve was linearly ranged from 2 to 2000 ng/mL (r2 ≥ 0.995).
The relative standard deviation (RSD) of interday precision and intraday precision was both lower than 15%, and the accuracy was between 91.7% and 108.0%.
The average recovery was >66.9%, and the matrix effects were from 104.8% to 111.0%.
In this assay, a fast, highly sensitive, and reproducible quantitative method was developed and validated in mouse blood for the first time.
The absolute availability of TBMS-I in the mouse was only 1%, exhibiting a poor oral absorption.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Lian-Guo& Weng, Qinghua& Li, Feifei& Liu, Jinlai& Zhang, Xueliang& Zhou, Yunfang. 2018. Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176650
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Lian-Guo…[et al.]. Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1176650
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Lian-Guo& Weng, Qinghua& Li, Feifei& Liu, Jinlai& Zhang, Xueliang& Zhou, Yunfang. Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176650
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176650
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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