Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS
Joint Authors
Liu, Jinlai
Weng, Qinghua
Li, Feifei
Zhang, Xueliang
Zhou, Yunfang
Chen, Lian-Guo
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of this study is to establish and validate a rapid, selective, and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine tubeimoside I (TBMS-I) in ICR (Institute of Cancer Research) mouse whole blood and its application in the pharmacokinetics and bioavailability study.
The blood samples were precipitated by acetonitrile to extract the analytes.
Chromatographic separation was performed on a UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm).
The mobile phase consisted of water with 0.1% formic acid and methanol (1 : 1, v/v) at a flow rate of 0.4 mL/min.
The total eluting time was 4 min.
The TBMS-I and ardisiacrispin A (internal standard (IS)) were quantitatively detected by a tandem mass spectrometry equipped with an electrospray ionization (ESI) in a positive mode by multiple reaction monitoring (MRM).
A validation of this method was in accordance with the US Food and Drug Administration (FDA) guidelines.
The lower limit of quantification (LLOQ) of TBMS-I was 2 ng/mL, and the calibration curve was linearly ranged from 2 to 2000 ng/mL (r2 ≥ 0.995).
The relative standard deviation (RSD) of interday precision and intraday precision was both lower than 15%, and the accuracy was between 91.7% and 108.0%.
The average recovery was >66.9%, and the matrix effects were from 104.8% to 111.0%.
In this assay, a fast, highly sensitive, and reproducible quantitative method was developed and validated in mouse blood for the first time.
The absolute availability of TBMS-I in the mouse was only 1%, exhibiting a poor oral absorption.
American Psychological Association (APA)
Chen, Lian-Guo& Weng, Qinghua& Li, Feifei& Liu, Jinlai& Zhang, Xueliang& Zhou, Yunfang. 2018. Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176650
Modern Language Association (MLA)
Chen, Lian-Guo…[et al.]. Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1176650
American Medical Association (AMA)
Chen, Lian-Guo& Weng, Qinghua& Li, Feifei& Liu, Jinlai& Zhang, Xueliang& Zhou, Yunfang. Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MSMS. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176650
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176650