Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood
المؤلفون المشاركون
Wen, Congcong
Fu, Huiyan
Jin, Yue
Shao, Lingjiu
Jin, Yongxi
Ma, Jianshe
Wang, Xianqin
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-10-11
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Delsoline, a major alkaloid of Delphinium anthriscifolium Hance, has both a curare-like effect and a ganglion-blocking effect and is used to relieve muscle tension or hyperkinesia.
A ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the determination of delsoline in mouse blood, and the pharmacokinetics of delsoline after intravenous administration (1 mg/kg) and intragastric administration (9, 6, and 3 mg/kg) were studied.
Gelsenicine served as an internal standard, and a UPLC BEH C18 chromatographic column was used.
The mobile phase consisted of acetonitrile and 0.1% formic acid; the gradient elution flow rate was 0.4 mL/min.
The MRM model was used for the quantitative analysis of delsoline m/z 468.3⟶108.1 and the internal standard m/z 327.1⟶296.1.
Mouse blood samples were treated with acetonitrile precipitation to remove proteins.
In the concentration range of 0.1–1000 ng/mL, delsoline in mouse blood showed a good linearity (r2 > 0.995), and the lower limit of quantitation was 0.1 ng/mL.
The intraday precision relative standard deviation (RSD) was below 14%, and the interday precision RSD was below 15%.
The accuracy ranged between 94.3% and 110.1%, the average recovery was above 90.8%, and the matrix effect ranged between 97.0% and 102.5%.
The UPLC-MS/MS method was sensitive, rapid, and selective in the study of pharmacokinetics of delsoline.
The absolute bioavailability of delsoline was 20.9%.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shao, Lingjiu& Jin, Yue& Fu, Huiyan& Ma, Jianshe& Wang, Xianqin& Jin, Yongxi…[et al.]. 2018. Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176662
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shao, Lingjiu…[et al.]. Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176662
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shao, Lingjiu& Jin, Yue& Fu, Huiyan& Ma, Jianshe& Wang, Xianqin& Jin, Yongxi…[et al.]. Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176662
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176662
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر