Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood
Joint Authors
Wen, Congcong
Fu, Huiyan
Jin, Yue
Shao, Lingjiu
Jin, Yongxi
Ma, Jianshe
Wang, Xianqin
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Delsoline, a major alkaloid of Delphinium anthriscifolium Hance, has both a curare-like effect and a ganglion-blocking effect and is used to relieve muscle tension or hyperkinesia.
A ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the determination of delsoline in mouse blood, and the pharmacokinetics of delsoline after intravenous administration (1 mg/kg) and intragastric administration (9, 6, and 3 mg/kg) were studied.
Gelsenicine served as an internal standard, and a UPLC BEH C18 chromatographic column was used.
The mobile phase consisted of acetonitrile and 0.1% formic acid; the gradient elution flow rate was 0.4 mL/min.
The MRM model was used for the quantitative analysis of delsoline m/z 468.3⟶108.1 and the internal standard m/z 327.1⟶296.1.
Mouse blood samples were treated with acetonitrile precipitation to remove proteins.
In the concentration range of 0.1–1000 ng/mL, delsoline in mouse blood showed a good linearity (r2 > 0.995), and the lower limit of quantitation was 0.1 ng/mL.
The intraday precision relative standard deviation (RSD) was below 14%, and the interday precision RSD was below 15%.
The accuracy ranged between 94.3% and 110.1%, the average recovery was above 90.8%, and the matrix effect ranged between 97.0% and 102.5%.
The UPLC-MS/MS method was sensitive, rapid, and selective in the study of pharmacokinetics of delsoline.
The absolute bioavailability of delsoline was 20.9%.
American Psychological Association (APA)
Shao, Lingjiu& Jin, Yue& Fu, Huiyan& Ma, Jianshe& Wang, Xianqin& Jin, Yongxi…[et al.]. 2018. Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176662
Modern Language Association (MLA)
Shao, Lingjiu…[et al.]. Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176662
American Medical Association (AMA)
Shao, Lingjiu& Jin, Yue& Fu, Huiyan& Ma, Jianshe& Wang, Xianqin& Jin, Yongxi…[et al.]. Pharmacokinetics and UPLC-MSMS of Delsoline in Mouse Whole Blood. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176662