Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material
المؤلفون المشاركون
Gaston, Joel D.
Bischel, Lauren L.
Fitzgerald, Lisa A.
Cusick, Kathleen D.
Ringeisen, Bradley R.
Pirlo, Russell K.
المصدر
Journal of Healthcare Engineering
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Disruption of the blood-brain barrier (BBB) is the hallmark of many neurovascular disorders, making it a critically important focus for therapeutic options.
However, testing the effects of either drugs or pathological agents is difficult due to the potentially damaging consequences of altering the normal brain microenvironment.
Recently, in vitro coculture tissue models have been developed as an alternative to animal testing.
Despite low cost, these platforms use synthetic scaffolds which prevent normal barrier architecture, cellular crosstalk, and tissue remodeling.
We created a biodegradable electrospun gelatin mat “biopaper” (BP) as a scaffold material for an endothelial/astrocyte coculture model allowing cell-cell contact and crosstalk.
To compare the BP and traditional models, we investigated the expression of 27 genes involved in BBB permeability, cellular function, and endothelial junctions at different time points.
Gene expression levels demonstrated higher expression of transcripts involved in endothelial junction formation, including TJP2 and CDH5, in the BP model.
The traditional model had higher expression of genes associated with extracellular matrix-associated proteins, including SPARC and COL4A1.
Overall, the results demonstrate that the BP coculture model is more representative of a healthy BBB state, though both models have advantages that may be useful in disease modeling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gaston, Joel D.& Bischel, Lauren L.& Fitzgerald, Lisa A.& Cusick, Kathleen D.& Ringeisen, Bradley R.& Pirlo, Russell K.. 2017. Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material. Journal of Healthcare Engineering،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1181081
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gaston, Joel D.…[et al.]. Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material. Journal of Healthcare Engineering No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1181081
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gaston, Joel D.& Bischel, Lauren L.& Fitzgerald, Lisa A.& Cusick, Kathleen D.& Ringeisen, Bradley R.& Pirlo, Russell K.. Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material. Journal of Healthcare Engineering. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1181081
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1181081
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر