Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material

Joint Authors

Gaston, Joel D.
Bischel, Lauren L.
Fitzgerald, Lisa A.
Cusick, Kathleen D.
Ringeisen, Bradley R.
Pirlo, Russell K.

Source

Journal of Healthcare Engineering

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-11-29

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Public Health
Medicine

Abstract EN

Disruption of the blood-brain barrier (BBB) is the hallmark of many neurovascular disorders, making it a critically important focus for therapeutic options.

However, testing the effects of either drugs or pathological agents is difficult due to the potentially damaging consequences of altering the normal brain microenvironment.

Recently, in vitro coculture tissue models have been developed as an alternative to animal testing.

Despite low cost, these platforms use synthetic scaffolds which prevent normal barrier architecture, cellular crosstalk, and tissue remodeling.

We created a biodegradable electrospun gelatin mat “biopaper” (BP) as a scaffold material for an endothelial/astrocyte coculture model allowing cell-cell contact and crosstalk.

To compare the BP and traditional models, we investigated the expression of 27 genes involved in BBB permeability, cellular function, and endothelial junctions at different time points.

Gene expression levels demonstrated higher expression of transcripts involved in endothelial junction formation, including TJP2 and CDH5, in the BP model.

The traditional model had higher expression of genes associated with extracellular matrix-associated proteins, including SPARC and COL4A1.

Overall, the results demonstrate that the BP coculture model is more representative of a healthy BBB state, though both models have advantages that may be useful in disease modeling.

American Psychological Association (APA)

Gaston, Joel D.& Bischel, Lauren L.& Fitzgerald, Lisa A.& Cusick, Kathleen D.& Ringeisen, Bradley R.& Pirlo, Russell K.. 2017. Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material. Journal of Healthcare Engineering،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1181081

Modern Language Association (MLA)

Gaston, Joel D.…[et al.]. Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material. Journal of Healthcare Engineering No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1181081

American Medical Association (AMA)

Gaston, Joel D.& Bischel, Lauren L.& Fitzgerald, Lisa A.& Cusick, Kathleen D.& Ringeisen, Bradley R.& Pirlo, Russell K.. Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material. Journal of Healthcare Engineering. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1181081

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1181081