Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid
المؤلفون المشاركون
Gao, Lili
Liu, Yuefeng
Zhang, Xiaoyu
Zhang, Hongsheng
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-04
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
2-Keto-L-gulonic acid (2-KLG), the direct precursor of vitamin C, is produced by a two-step fermentation route from D-sorbitol in industry.
However, this route is a complicated mix-culture system which involves three bacteria.
Thus, replacement of the conventional two-step fermentation process with a one-step process could be revolutionary in vitamin C industry.
The one-step fermentation of 2-keto-L-gulonic acid (2-KLG) has been achieved in our previous study; 32.4 g/L of 2-KLG production was obtained by the one-step strain G.
oxydans/pGUC-tufB-sdh-GGGGS-sndh after 168 h.
In this study, L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH) were expressed in G.
oxydans after the codon optimization.
Furthermore, the trimeric protein CutA was used to improve the chemical structure stability of SDH and SNDH.
The recombinant strain G.
oxydans/pGUC-tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA produced 40.3 g/L of 2-KLG after 168 h.
In addition, the expression levels of the cofactor PQQ were enhanced to further improve 2-KLG production.
With the stepwise metabolic engineering of G.
oxydans, the final 2-KLG production was improved to 42.6 g/L.
The efficient one-step production of 2-KLG was achieved, and the final one-step industrial-scale production of 2-KLG is drawing near.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Lili& Liu, Yuefeng& Zhang, Xiaoyu& Zhang, Hongsheng. 2020. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181889
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Lili…[et al.]. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1181889
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Lili& Liu, Yuefeng& Zhang, Xiaoyu& Zhang, Hongsheng. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181889
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1181889
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر